Gut Microbiota Mediate Insecticide Resistance in the Diamondback Moth, Plutella xylostella (L.)
نویسندگان
چکیده
The development of insecticide resistance in insect pests is a worldwide concern and elucidating the underlying mechanisms is critical for effective crop protection. Recent studies have indicated potential links between insect gut microbiota and insecticide resistance and these may apply to the diamondback moth, Plutella xylostella (L.), a globally and economically important pest of cruciferous crops. We isolated Enterococcus sp. (Firmicutes), Enterobacter sp. (Proteobacteria), and Serratia sp. (Proteobacteria) from the guts of P. xylostella and analyzed the effects on, and underlying mechanisms of insecticide resistance. Enterococcus sp. enhanced resistance to the widely used insecticide, chlorpyrifos, in P. xylostella, while in contrast, Serratia sp. decreased resistance and Enterobacter sp. and all strains of heat-killed bacteria had no effect. Importantly, the direct degradation of chlorpyrifos in vitro was consistent among the three strains of bacteria. We found that Enterococcus sp., vitamin C, and acetylsalicylic acid enhanced insecticide resistance in P. xylostella and had similar effects on expression of P. xylostella antimicrobial peptides. Expression of cecropin was down-regulated by the two compounds, while gloverin was up-regulated. Bacteria that were not associated with insecticide resistance induced contrasting gene expression profiles to Enterococcus sp. and the compounds. Our studies confirmed that gut bacteria play an important role in P. xylostella insecticide resistance, but the main mechanism is not direct detoxification of insecticides by gut bacteria. We also suggest that the influence of gut bacteria on insecticide resistance may depend on effects on the immune system. Our work advances understanding of the evolution of insecticide resistance in this key pest and highlights directions for research into insecticide resistance in other insect pest species.
منابع مشابه
DNA Sequencing Reveals the Midgut Microbiota of Diamondback Moth, Plutella xylostella (L.) and a Possible Relationship with Insecticide Resistance
BACKGROUND Insect midgut microbiota is important in host nutrition, development and immune response. Recent studies indicate possible links between insect gut microbiota and resistance to biological and chemical toxins. Studies of this phenomenon and symbionts in general have been hampered by difficulties in culture-based approach. In the present study, DNA sequencing was used to examine the mi...
متن کاملDevelopment of genetic markers to study dispersal of Diamondback moth, Plutella xylostella (L.) in Australia
The origin and insecticide resistance status of Diamondback moth (Plutella xylostella) populations infesting canola, vegetables, forage brassicas and weeds has fundamental implications for management of the pest in Australia. Diamondback moth is considered a migratory species in the Northern Hemisphere, but although there is evidence that Diamondback moth moves en-masse throughout Australia in ...
متن کاملInsecticide Resistance in Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) in the Federal District, Brazil
The levels of resistance to the insecticides cartap, deltamethrin and metamidophos were evaluated for three populations of the Diamondback Moth Plutella xylostella (L.) from the Federal District, Brazil in laboratory bioassays. The larvae of DBM showed levels of resistance to deltamethrin between 4 and 47 fold relative to a susceptible laboratory strain. The level of resistance to metamidophos ...
متن کاملInsecticide resistance in diamondback moth (DBM), Plutella xylostella (L.): status and prospects for its management in India
Insecticide resistance and concomittant field failure to control the diamondback moth (DBM), Plutella xylostella were first recorded in 1968 in Punjab. Studies carried out in different states like Tamil Nadu, Karnataka, Madhya Pradesh, Punjab, indicate that fenvalerate, quilnalphos and monocrotophos resistance are now ubiquitous in Plutella xylostella. In Tamil Nadu, the discriminating dose tec...
متن کاملDeltamethrin resistance in some diamondback moth, Plutella xylostella populations in Iran
The diamondback moth, Plutella xylostella has been developed resistance to many groups of pesticides including of pyrethroids. The present study was conducted to evaluate the toxicity of deltamethrin on the third instar larvae of six populations of the pest using leaf dipping method. The results showed that different populations had different susceptibilities to deltamethrin. At the LC50 level,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 9 شماره
صفحات -
تاریخ انتشار 2018